| Lightning Social VenturesFintech | Fintech | London, United Kingdom | — | lightningreach.org |
| LightstreamOther | Other | Chicago, United States | — | golightstream.com |
| LightwingB2B | B2B | Bengaluru, India | Aurélie Hélouis | lightwing.io |
| LilacB2B | B2B | San Francisco, United States | | getlilac.com |
| LimboHealthcare | Healthcare | — | — | limborevolution.com |
| Lindus HealthHealthcare | Healthcare | — | Meri Beckwith | lindushealth.com |
| LinearB2B | B2B | — | Karri Saarinen | linear.app |
| LineSightOther | Other | Oak Brook, United States | — | linesight-ai.com |
| Lingco Language LabsEducation | Education | Lansing, United States | Robert Sherwood | lingco.io |
| LingvistB2B | B2B | Tallinn, Estonia | Mait Müntel | lingvist.com |
| LinkBycarB2B | B2B | Paris, France | — | linkbycar.com |
| Linker FinanceFintech | Fintech | Pasadena, United States | — | linkerfinance.com |
| LinkupB2B | B2B | — | — | linkup.so |
| LinqAlphaOther | Other | Cambridge, United States | — | linqalpha.com |
| LipefiFintech | Fintech | Elsene, Belgium | — | lipefi.com |
| LiquidOther | Other | Los Angeles, United States | — | goliquid.io |
| Liquid DiamondsFintech | Fintech | New York City, United States | — | liquid.diamonds |
| LISNRB2B | B2B | Cincinnati, United States | Rodney Williams | lisnr.com |
| LiteStackFintech | Fintech | San Antonio, United States | — | zerovm.org |
| LitographsOther | Other | Cambridge, United States | James Dowd | litographs.com |
| LivOther | Other | San Francisco, United States | — | liv.tv |
| LIVConsumer | Consumer | — | — | liv.tv |
| Liva AIB2B | B2B | San Francisco, United States | | theliva.ai |
| LiveblocksB2B | B2B | — | Guillaume Salles | liveblocks.io |
| LiveFlowFintech | Fintech | — | Lasse Kalkar | liveflow.com |
| LiveLikeConsumer | Consumer | New York City, United States | Miheer Walavalkar | livelikevr.com |
| Liveoak TechnologiesB2B | B2B | Austin, United States | Will Thompkins | liveoak.net |
| Livsn DesignsOther | Other | Fayetteville, United States | Eric Browne | livsndesigns.com |
| Lo BioHealthcare | Healthcare | Rockville, United States | — | lo-bio.com |
| loc.taxB2B | B2B | — | — | loctax.com |
| Local CrateIndustrials | Industrials | Chanhassen, United States | — | localcratemeals.com |
| LocalyticsConsumer | Consumer | Boston, United States | Raj Aggarwal | localytics.com |
| LocataHealthcare | Healthcare | New York, United States | | locatahealth.com |
| LocateAIOther | Other | San Francisco, United States | — | locate.ai |
| LocusB2B | B2B | — | | paywithlocus.com |
| LodgifyConsumer | Consumer | — | Dennis Klett | lodgify.com |
| LogicalB2B | B2B | San Francisco, United States | Sam Karu | logical.io |
| LogicGateOther | Other | Chicago, United States | Matt Kunkel | logicgate.com |
| LogixboardB2B | B2B | Seattle, United States | Julian Alvarez | logixboard.com |
| LogosGuardB2B | B2B | San Francisco, United States | | logosguard.com |
| LookerB2B | B2B | — | Lloyd Tabb | looker.com |
| LoopB2B | B2B | United States | — | tryloop.ai |
| LorisB2B | B2B | Columbus, United States | Ethan Aaron | getloris.com |
| LosantOther | Other | Cincinnati, United States | Heath Doerr | losant.com |
| LotasB2B | B2B | San Francisco, United States | | lotas.ai |
| LOU Web TechnologiesEducation | Education | New York City, United States | — | louassist.com |
| LouisOther | Other | Labège, France | — | louis.design |
| Louiza LabsB2B | B2B | San Francisco, United States | | louizalabs.co |
| Lounn MXFintech | Fintech | HIPODROMO-CUAUHTEMOC, Mexico | — | lounn.mx |
| LovableB2B | B2B | Sweden | Anton Osika | lovable.dev |