| LabricB2B | B2B | — | | labric.co |
| LabstepB2B | B2B | — | Jonathan Starkie | labstep.com |
| Lactation LabOther | Other | Santa Monica, United States | Stephanie Canale | lactationlab.com |
| LagoonFintech | Fintech | Tel Aviv, Israel | — | data-lagoon.com |
| LaiblOther | Other | Halifax, Canada | — | laibl.co |
| LakoniaGovernment | Government | San Francisco, United States | | lakonia.us.com |
| Lamina LabsB2B | B2B | — | | laminalabs.ai |
| LanceB2B | B2B | — | | lance.live |
| Land InsightConsumer | Consumer | — | Jonny Anstead | land.tech |
| LandngB2B | B2B | Honolulu, United States | — | landng.com |
| LaneOther | Other | Toronto, Canada | Clinton Robinson | joinlane.com |
| LanesurfB2B | B2B | San Francisco, United States | | lanesurf.com |
| LapelB2B | B2B | — | | lapel.com |
| LapisB2B | B2B | San Francisco, United States | Varunram Ganesh | trylapis.com |
| LarkB2B | B2B | San Francisco, United States | | getlark.ai |
| LatchOther | Other | New York City, United States | Luke Schoenfelder | latch.com |
| LateralB2B | B2B | — | Marc Verstaen | lateral.io |
| Latimer ControlsB2B | B2B | Boulder, United States | — | latimercontrols.com |
| Latitudo40Industrials | Industrials | Naples, Italy | Alessio Traficante | latitudo40.com |
| LaurenceB2B | B2B | — | | trylaurence.com |
| Lauretta.ioB2B | B2B | Singapore, Singapore | — | lauretta.io |
| LawnStarterConsumer | Consumer | Austin, United States | Ryan Farley | lawnstarter.com |
| LayUp TechnologiesFintech | Fintech | Johannesburg, South Africa | Lethiwe Dube | layup.co.za |
| LeadbayB2B | B2B | San Francisco, United States | | leadbay.ai |
| Lean Startup MachineB2B | B2B | San Francisco, United States | Trevor Owens | javelin.com |
| LeanplumOther | Other | San Francisco, United States | Momchil Kyurkchiev | leanplum.com |
| LearnPlatformOther | Other | Raleigh, United States | Ed Brophy | learnplatform.com |
| Legaleaze TechnologiesOther | Other | San Francisco, United States | — | legaleaze.co |
| LegalOSB2B | B2B | — | | legalos.ai |
| LegalPadB2B | B2B | Seattle, United States | Tahmina Watson | legalpad.io |
| Legit PatentsB2B | B2B | — | — | legit.ai |
| LegitFitFintech | Fintech | Cork, Ireland | — | legitfit.com |
| LemmaB2B | B2B | San Francisco, United States | | uselemma.ai |
| Lena HealthB2B | B2B | Houston, United States | — | lena.io |
| LendAPIFintech | Fintech | Orange, United States | Victor Cheng | lendapi.com |
| LendKoiFintech | Fintech | Sunrise, United States | — | lendkoi.com |
| LendorseFintech | Fintech | Munich, Germany | — | lendorse.com |
| LeOOther | Other | Tel Aviv, Israel | — | meetleo.co |
| Let's EnhanceB2B | B2B | Kyiv, Ukraine | Alex Savsunenko | letsenhance.io |
| LeucineB2B | B2B | Koramangala 7th Block, India | Rahul Gonuguntla | leucinetech.com |
| Level2Fintech | Fintech | Cardiff, United Kingdom | — | trylevel2.com |
| LevelTen EnergyIndustrials | Industrials | Seattle, United States | Bryce Smith | leveltenenergy.com |
| LexiB2B | B2B | San Francisco, United States | | getlexi.io |
| LexiusB2B | B2B | — | | lexius.ai |
| Librar LabsB2B | B2B | San Francisco, United States | Jonathan Görtz | librarlabs.com |
| LibryoB2B | B2B | — | Garth Willis | libryo.com |
| Lifebit BiotechOther | Other | London, United Kingdom | Maria Chatzou Dunford | lifebit.ai |
| LightFintech | Fintech | — | Nicolas Bailly | light.inc |
| LightberryIndustrials | Industrials | San Francisco, United States | | lightberry.com |
| LightboxB2B | B2B | San Mateo, United States | — | ninesixteen.com |