| Damon MotorcyclesOther | Other | Vancouver, Canada | Jay Giraud | damon.com |
| Dapple SecurityB2B | B2B | Centennial, United States | Dhrumil Shah | dapplesecurity.com |
| Dara.networkB2B | B2B | Bengaluru, India | — | dara.network |
| DataboxOther | Other | Boston, United States | Peter Caputa | databox.com |
| DataCampEducation | Education | New York City, United States | Martijn Theuwissen | datacamp.com |
| DataradeB2B | B2B | Berlin, Germany | Johannes Reck | datarade.ai |
| DataRobotB2B | B2B | Boston, United States | Jeremy Achin | datarobot.com |
| DataSineB2B | B2B | London, United Kingdom | Igor Volzhanin | datasine.com |
| DatawispConsumer | Consumer | Hempstead, United States | — | datawisp.io |
| DauplerB2B | B2B | Kansas City, United States | — | daupler.com |
| De-IceIndustrials | Industrials | Somerville, United States | — | deice.io |
| Dear Doc SingaporeHealthcare | Healthcare | Gurgaon, India | — | dear-doc.com |
| Decorte Future IndustriesOther | Other | Cambridge, United Kingdom | — | decortefutureindustries.com |
| Deep 6 AIB2B | B2B | Pasadena, United States | Wout Brusselaers | deep6.ai |
| Deepflow AIB2B | B2B | Bordeaux, France | — | deepflow.ai |
| DeepHowB2B | B2B | New York City, United States | Sam Zheng | deephow.com |
| DeepStream TechnologiesB2B | B2B | London, United Kingdom | Rob Lamb | deepstreamtech.com |
| DegreedEducation | Education | San Francisco, United States | David Blake | degreed.com |
| DeliverartOther | Other | Rome, Italy | — | deliverart.it |
| DemandlyOther | Other | Miami, United States | — | demandly.com |
| DenimFintech | Fintech | New York City, United States | Jim Granat | denim.com |
| DepayFintech | Fintech | Buenos Aires, Argentina | — | depayapp.com |
| DerqB2B | B2B | Dubai, United Arab Emirates | Georges Aoude | derq.com |
| DfnsFintech | Fintech | Paris, France | Nicolas Bacca | dfns.co |
| Diagon TechnologiesOther | Other | Alameda, United States | — | diagon.ai |
| DidimoOther | Other | Leca Da Palmeira, Portugal | Verónica Orvalho | mydidimo.com |
| DiemOther | Other | New York City, United States | Meghna Dholakia | askdiem.com |
| Dig RoboticsIndustrials | Industrials | Nir Am, Israel | — | digrobotics.com |
| DigiseqFintech | Fintech | London, United Kingdom | Terrence Swanson | digiseq.co.uk |
| DigitalOceanB2B | B2B | New York City, United States | Ben Uretsky | digitalocean.com |
| DimeqOther | Other | Kalvåg, Norway | — | dimeq.no |
| DirecTech LabsB2B | B2B | Santa Monica, United States | — | directechlabs.com |
| Dispatch GoodsIndustrials | Industrials | San Francisco, United States | Lindsey Hoell | dispatchgoods.com |
| Distil NetworksB2B | B2B | Arlington, United States | Rami Essaid | distilnetworks.com |
| DLeaderHealthcare | Healthcare | Osaka, Japan | — | dleader.co.jp |
| DLT PaymentsFintech | Fintech | Tallinn, Estonia | — | dltpay.com |
| DNSFilterB2B | B2B | Washington, United States | Ken Carnesi | dnsfilter.com |
| DocStationHealthcare | Healthcare | Austin, United States | Alex Barker | docstation.co |
| docTrackrB2B | B2B | Cambridge, United States | Inès Nasri | doctrackr.com |
| DocuMentorHealthcare | Healthcare | Indianapolis, United States | — | yourdocumentor.com |
| doDOCOther | Other | Boston, United States | Rui Oliveira | dodoc.com |
| DollarizeFintech | Fintech | Brooklyn, United States | — | dollarize.me |
| Donut MediaOther | Other | Los Angeles, United States | Matt Levatich | donut.media |
| DoorrFintech | Fintech | Nobleton, Canada | — | doorr.com |
| dopayB2B | B2B | London, United Kingdom | Brian Breakfast | dopay.com |
| dopl technologiesHealthcare | Healthcare | Bothell, United States | — | dopltechnologies.com |
| DotterelOther | Other | Auckland, New Zealand | — | dotterel.co.nz |
| DownstreamB2B | B2B | Seattle, United States | — | downstreamimpact.com |
| Dr.OnlineHealthcare | Healthcare | Caparica, Portugal | — | dronline.com |
| DraftedOther | Other | Miami, United States | Vinayak Ranade | joindrafted.com |