| Surgicure TechnologiesHealthcare | Healthcare | Charlestown, United States | — | surgicuretech.com |
| SurPlusMapOther | Other | Oslo, Norway | — | surplusmap.com |
| SURVIVROther | Other | Richardson, United States | — | survivr.com |
| SustainSoftConsumer | Consumer | Paris, France | — | sustainsoft.eu |
| Swag.comOther | Other | New York City, United States | Jeremy Parker | swag.com |
| SweatcoinFintech | Fintech | — | Oleg Fomenko | sweatco.in |
| SwiftComplyB2B | B2B | Dublin, Ireland | — | swiftcomply.io |
| SwiftShiftHealthcare | Healthcare | — | — | swiftshift.com |
| SwingVisionOther | Other | Milpitas, United States | Swupnil Sahai | swing.tennis |
| Switchboard LiveOther | Other | Philadelphia, United States | Drew Stretch | switchboard.live |
| swivlB2B | B2B | Denver, United States | — | tryswivl.com |
| SwooveOther | Other | New York City, United States | — | swoove.delivery |
| SwordHealthcare | Healthcare | — | Virgilio Bento | sword-health.com |
| SworkitIndustrials | Industrials | Bethesda, United States | Ryan Hanna | sworkit.com |
| SwymConsumer | Consumer | Seattle, United States | Praveen Krishnamurthy | getswym.com |
| Sygaldry TechnologiesIndustrials | Industrials | — | | sygaldry.com |
| SylloTipsB2B | B2B | Rome, Italy | — | syllotips.com |
| SylveraIndustrials | Industrials | — | Allister Furey | sylvera.com |
| Symbl.aiB2B | B2B | San Jose, United States | Surbhi Rathore | rammer.ai |
| SympliFiFintech | Fintech | London, United Kingdom | — | symplifi.co.uk |
| SynackOther | Other | Redwood City, United States | Jay Kaplan | synack.com |
| SyncOnSet TechnologiesOther | Other | Los Angeles, United States | Vera Bobinaite | synconset.com |
| SynthesiaB2B | B2B | — | Victor Riparbelli | synthesia.io |
| SynthesizedB2B | B2B | — | Nicolai Baldin | synthesized.io |
| Synthetic SciencesB2B | B2B | — | | syntheticsciences.ai |
| Synthetic SocietyB2B | B2B | San Francisco, United States | | syntheticsociety.ai |
| SyntropyB2B | B2B | — | | syntropy.io |
| TablenderB2B | B2B | New York City, United States | — | tablender.com |
| tagSpaceOther | Other | Byron Bay, Australia | — | tagspace.com |
| TaizenB2B | B2B | Tallinn, Estonia | — | usetaizen.com |
| Take2.aiB2B | B2B | New York City, United States | — | take2.ai |
| TakeShapeB2B | B2B | Brooklyn, United States | Andrew Sprouse | takeshape.io |
| TalasimConsumer | Consumer | — | — | jeeran.com |
| TalentQLB2B | B2B | Round Rock, United States | Opeyemi Awosika | talentql.com |
| Talimai SASEducation | Education | Orléans, France | — | brio.ink |
| TalivestB2B | B2B | Dublin, Ireland | Roly Walter | talivest.com |
| Talk-A-BotB2B | B2B | Budapest, Hungary | — | talkabot.net |
| Talking ComputersB2B | B2B | — | | talkingcomputers.ai |
| TalkPushB2B | B2B | — | Max Armbruster | talkpush.com |
| TalkstackB2B | B2B | Singapore, Singapore | — | talkstack.ai |
| TanazaB2B | B2B | — | Sebastiano Bertani | tanaza.com |
| TangibleeB2B | B2B | Chicago, United States | Ori Marku | tangiblee.com |
| TanoB2B | B2B | — | — | tano.ai |
| taptrip.coIndustrials | Industrials | London, United Kingdom | — | taptrip.co |
| TaqticB2B | B2B | Australia | — | tactiq.app |
| Target ArmOther | Other | Ridgefield, United States | — | targetarm.com |
| TassoHealthcare | Healthcare | Seattle, United States | Ben Casavant | tassoinc.com |
| TatioB2B | B2B | Tel Aviv, Israel | Gal Hen | tatio.io |
| TattvamB2B | B2B | — | — | tattvamlabs.ai |
| TawkitAIB2B | B2B | Issaquah, United States | Atai Barkai | copilotkit.ai |