| NixtlaOther | Other | Mexico City, Mexico | Max Mergenthaler Canseco | nixtla.io |
| NoCodeOther | Other | Denver, United States | — | enzyme.so |
| Nodes and LinksB2B | B2B | — | Cristian Dorobantescu | nodeslinks.com |
| nodes.gardenFintech | Fintech | Dover, United States | — | nodes.garden |
| NoledgeLossOther | Other | Tel Aviv, Israel | — | noledgeloss.com |
| NomiB2B | B2B | San Francisco, United States | Swan Beaujard | nomi.so |
| NordigenFintech | Fintech | — | Rolands Mesters | nordigen.com |
| NoriHealthcare | Healthcare | New York, United States | | nori.ai |
| NormalIndustrials | Industrials | San Francisco, United States | | normalfactory.com |
| NorraHealthcare | Healthcare | San Francisco, United States | | norra.io |
| NOSO LABSIndustrials | Industrials | — | | noso.so |
| Nossa DataB2B | B2B | London, United Kingdom | — | nossadata.com |
| NotchOther | Other | Cambridge, United States | — | notchtechnologies.com |
| NotedsourceB2B | B2B | New York City, United States | Chris Lynch | notedsource.io |
| Noteworthy AIOther | Other | Stamford, United States | — | noteworthy.ai |
| NotionB2B | B2B | — | Ivan Zhao | notion.so |
| NotionOther | Other | Denver, United States | — | getnotion.com |
| NotteB2B | B2B | San Francisco, United States | | notte.cc |
| nouriHealthcare | Healthcare | New York City, United States | — | heynouri.com |
| NovaflowHealthcare | Healthcare | San Francisco, United States | | novaflowapp.com |
| NovaliaOther | Other | Cambridge, United Kingdom | Kate Stone | novalia.co.uk |
| Novel EffectFintech | Fintech | Seattle, United States | Matt Sandoval | noveleffect.com |
| NovicapFintech | Fintech | London, United Kingdom | Nicolas Overloop | novicap.com |
| NovoB2B | B2B | New York City, United States | Michael Rangel | novo.co |
| Novo SpaceOther | Other | Cambridge, United States | Yariv Geller | novo.space |
| NovoflowHealthcare | Healthcare | — | | novoflow.io |
| Nox MetalsIndustrials | Industrials | Detroit, United States | Zane Hengsperger | noxmetals.co |
| NozomioB2B | B2B | San Francisco, United States | | trynia.ai |
| NozzleB2B | B2B | Lehi, United States | Brandon Checketts | nozzle.io |
| NubankFintech | Fintech | — | David Vélez | nubank.com.br |
| NucleoHealthcare | Healthcare | — | | nucleoresearch.com |
| Null LabsIndustrials | Industrials | San Francisco, United States | | n0labs.com |
| NurtureConsumer | Consumer | — | — | nurture.is |
| NutriSenseHealthcare | Healthcare | Chicago, United States | Kara Collier | nutrisense.io |
| O-IDOther | Other | Suginami, Japan | — | o-id.net |
| OB2 AIB2B | B2B | Wilmington, United States | — | ob2.ai |
| ObertOther | Other | Santa Monica, United States | — | obertworkwear.com |
| OBEYOther | Other | London, United Kingdom | — | obeyme.world |
| ObjectBoxOther | Other | Munich, Germany | Vivien Dollinger | objectbox.io |
| OctaPulseIndustrials | Industrials | — | | tryoctapulse.com |
| OdaptosOther | Other | Perols, France | — | odaptos.com |
| oDocConsumer | Consumer | Colombo, Sri Lanka | Chamila Magalagama | odoc.life |
| ODWENOther | Other | Noida, India | — | odwen.co.in |
| Odysseus Health AIB2B | B2B | New York City, United States | — | odysseushealthai.com |
| OE Systems ABB2B | B2B | Göteborg, Sweden | — | oe-systems.com |
| OfflineOther | Other | Chicago, United States | — | offlinetalent.com |
| OffrOther | Other | Dublin, Ireland | Rob Dolan | offr.io |
| OkibiB2B | B2B | San Francisco, United States | | okibi.ai |
| OkkularOther | Other | Melbourne, Australia | — | okkular.io |
| OKOIndustrials | Industrials | Luxembourg, Luxembourg | — | oko.finance |