| Tzuka LimitedOther | Other | Loughborough, United Kingdom | — | tzuka.com |
| UBIO Automation CloudOther | Other | London, United Kingdom | — | ubio.ai |
| Uhura SolutionsB2B | B2B | London, United Kingdom | — | uhurasolutions.com |
| Ultimate.aiOther | Other | Helsinki, Finland | Reetu Kainulainen | ultimate.ai |
| UnephraHealthcare | Healthcare | San Jose, United States | — | unephra.com |
| Unidentified Landed ObjectOther | Other | Los Angeles, United States | — | ulo.world |
| Unified TrackB2B | B2B | Knoxville, United States | — | unifiedtrack.ai |
| University StartupsEducation | Education | Gaithersburg, United States | — | university-startups.com |
| UnmadeB2B | B2B | London, United Kingdom | Hal Watts | unmade.com |
| unmessOther | Other | Reading, United Kingdom | — | tryrice.com |
| Unreal EstateOther | Other | Chicago, United States | Joe Pirelli | unrealestate.com |
| Unruly StudiosConsumer | Consumer | Jamaica Plain, United States | Ilana Shabtay | unruly-studios.com |
| UntiedFintech | Fintech | Surrey, United Kingdom | Kevin Sefton | untied.io |
| UpkidEducation | Education | Eagle Mountain, United States | — | joinupkid.com |
| UpsideHoMHealthcare | Healthcare | Fort Lauderdale, United States | — | joinupside.com |
| UpsieConsumer | Consumer | Burnsville, United States | Clarence Bethea | upsie.com |
| UptimeHealthHealthcare | Healthcare | Boston, United States | Oakley Kozloski | uptimehealth.com |
| Upward FinancialFintech | Fintech | Seattle, United States | — | upwardli.com |
| Urban SDKB2B | B2B | Atlantic Beach, United States | Jason Barnes | urbansdk.com |
| Urban SkyOther | Other | Denver, United States | Michael Wojcik | urbansky.com |
| UrbanFoxB2B | B2B | Dublin, Ireland | — | urbanfox.io |
| UtivaEducation | Education | Greenbelt, United States | Ope Adeoye | utiva.co |
| UTwinOther | Other | Turin, Italy | — | utwin.it |
| VakeB2B | B2B | Oslo, Norway | — | vake.ai |
| VakyaB2B | B2B | Mather, United States | — | vakya.ai |
| Valence DiscoveryB2B | B2B | Montréal, Canada | Joseph Kleinhenz | valencediscovery.com |
| Valene HealthB2B | B2B | New York City, United States | — | valenehealth.com |
| VALID8 FinancialB2B | B2B | Boulder, United States | — | valid8financial.com |
| ValidatedConsumer | Consumer | Seattle, United States | — | validated.co |
| ValueCoB2B | B2B | Paris, France | — | valuecometrics.com |
| ValyuuB2B | B2B | Gorinchem, Netherlands | — | valyuu.com |
| Van RoboticsB2B | B2B | Columbia, United States | AnnMarie Thomas | smartrobottutor.com |
| VanHackB2B | B2B | Vancouver, Canada | Ilya Brotzky | vanhack.com |
| VartegaIndustrials | Industrials | Golden, United States | Andrew Maxey | vartega.com |
| VascAIB2B | B2B | Sacramento, United States | — | vascai.com |
| Vector.aiOther | Other | London, United Kingdom | Zeki Turedi | raft.ai |
| VeendFintech | Fintech | Sheridan, United States | — | veendhq.com |
| VeesualB2B | B2B | Paris, France | Maxime Patte | veesual.ai |
| VehoIndustrials | Industrials | Boulder, United States | Itamar Zur | shipveho.com |
| VelociaIndustrials | Industrials | Toronto, Canada | — | velocia.io |
| VendorHawkOther | Other | Seattle, United States | — | vendorhawk.co |
| VenteurOther | Other | Berkeley, United States | Torben Nielsen | venteur.co |
| VerbalB2B | B2B | Covina, United States | — | tryverbal.com |
| VerbalizeItFintech | Fintech | New York City, United States | Ryan Frankel | verbalizeit.com |
| VerbzB2B | B2B | San Francisco, United States | — | verbz.ai |
| Verifi Data Corp.Industrials | Industrials | Houston, United States | — | verifi.eco |
| Verificient TechnologiesB2B | B2B | New York City, United States | Ujjwal Singh | verificient.com |
| VeriTXFintech | Fintech | Buffalo, United States | — | vertix.co |
| VermeerOther | Other | Queens, United States | — | getvermeer.com |
| VertConsumer | Consumer | Tel Aviv, Israel | — | vert.earth |