| ArweaveOther | Other | London, United Kingdom | Sam Williams | arweave.org |
| AsanifyFintech | Fintech | Kolkata, India | Ruchit Garg | asanify.com |
| AsephaB2B | B2B | Toronto, Canada | — | asepha.ai |
| AskSAMIEHealthcare | Healthcare | Kansas City, United States | — | asksamie.com |
| AskSid Conversational AIB2B | B2B | Bengaluru, India | Sid Banerjee | asksid.ai |
| Aspect HealthHealthcare | Healthcare | London, United Kingdom | Briana Christophers | aspect-health.com |
| AspinityOther | Other | Morgantown, United States | Tom Doyle | aspinity.com |
| AssemblrFintech | Fintech | Bandung, Indonesia | Hasbi Asyadiq | assemblrworld.com |
| Asset RealityFintech | Fintech | London, United Kingdom | Aidan Larkin | assetreality.com |
| AssetVaultB2B | B2B | London, United Kingdom | — | asset-vault.com |
| ASTRA by Czar SecuritiesB2B | B2B | Claymont, United States | — | getastra.com |
| AstroOther | Other | New York City, United States | — | hellotwist.com |
| Asvin TechB2B | B2B | Patna, India | — | dentratech.com |
| AteiosIndustrials | Industrials | La Jolla, United States | — | ateios.com |
| AthionB2B | B2B | Chesterfield, United States | — | athion.io |
| AthleteOther | Other | Harrogate, United Kingdom | — | lifeathlete.co |
| Atlas WearablesOther | Other | Austin, United States | Peter Li | atlaswearables.com |
| Atlas5DHealthcare | Healthcare | Cambridge, United States | — | atlas5d.com |
| ATMOIndustrials | Industrials | Berlin, Germany | — | atmo.earth |
| ATOMB2B | B2B | Greenville, United States | — | atomchat.io |
| Atomos SpaceIndustrials | Industrials | Denver, United States | Vanessa Clark | atomosspace.com |
| Audigo LabsOther | Other | San Francisco, United States | — | audigolabs.com |
| AUDIT XPRTB2B | B2B | London, United Kingdom | — | auditxprt.com |
| AuditocityB2B | B2B | Fairhope, United States | — | auditocity.com |
| AugurOther | Other | Boulder, United States | Jack Peterson | augur.io |
| AumetHealthcare | Healthcare | Amman, Jordan | Nasser Alsharif | aumet.me |
| Aunt FlowOther | Other | Columbus, United States | Claire Coder | goauntflow.com |
| AuquanB2B | B2B | London, United Kingdom | Radhika Subramanian | auquan.com |
| Aura FinanceEducation | Education | San Francisco, United States | — | aurafinance.io |
| Auron SolutionsB2B | B2B | Sunnyvale, United States | — | auron.solutions |
| AutobonOther | Other | Chicago, United States | — | autobon.ai |
| AutomotusIndustrials | Industrials | Los Angeles, United States | Aaron Dagon | automotus.co |
| AutonomOther | Other | Columbus, United States | — | autonom.cc |
| Auxilius.aiOther | Other | Unterföhring, Germany | — | auxilius.ai |
| Avarie FemcareOther | Other | Long Beach, United States | — | avariefemcare.com |
| AvenewsIndustrials | Industrials | Tel Aviv, Israel | Ronen Bensimon | avenews-gt.com |
| AVENIR TECHB2B | B2B | London, United Kingdom | — | joinavenir.com |
| Avido AIFintech | Fintech | Klampenborg, Denmark | — | avidoai.com |
| AvvayOther | Other | Nashville, United States | — | avvay.com |
| Aware HealthOther | Other | Dover, United States | — | awarehealth.io |
| AxccesOther | Other | Brooklyn, United States | — | axcces.com |
| Ayana TherapyOther | Other | Los Angeles, United States | Amira Barger | ayanatherapy.com |
| AZmedB2B | B2B | Paris, France | Alexandre Datry | azmed.co |
| AzoraOther | Other | San Francisco, United States | — | azora.space |
| AzuquaOther | Other | Seattle, United States | Cord Hamrick | azuqua.com |
| BabblerConsumer | Consumer | Paris, France | — | heybabbler.com |
| BackhouseOther | Other | New York City, United States | — | backhouseshop.com |
| Backpack HealthcareHealthcare | Healthcare | Elkridge, United States | — | youmehealthcare.com |
| BalanceFintech | Fintech | Toronto, Canada | — | balance.ca |
| BallBoxB2B | B2B | Ann Arbor, United States | — | playballbox.com |