| ImpalaConsumer | Consumer | London, United Kingdom | Ben Stephenson | impala.travel |
| ImpersonB2B | B2B | Culver City, United States | Eyal Pfeifel | imperson.com |
| ImproovyOther | Other | Chicago, United States | Jake Kahana | improovy.com |
| IMRSVOther | Other | New York City, United States | — | imrsv.com |
| INBOTIQAOther | Other | London, United Kingdom | Ulrika Jägare | inbotiqa.com |
| InclusivvOther | Other | Atlanta, United States | Jeremy Balkin | inclusivv.co |
| Indico DataB2B | B2B | Boston, United States | Slater Victoroff | indico.io |
| IndieFlowOther | Other | Santa Monica, United States | — | indieflow.me |
| Induction Food SystemsIndustrials | Industrials | Durham, United States | — | inductionfoodsystems.com |
| InfinitB2B | B2B | Paris, France | — | infinit.sh |
| Infinite Composites TechnologiesIndustrials | Industrials | Tulsa, United States | Eric Lund | infinitecomposites.com |
| Influunt SystemsIndustrials | Industrials | Barcelona, Spain | — | influunt.ai |
| InjoyOther | Other | Montréal, Canada | — | injoy.bio |
| Inkblot TherapyOther | Other | Burlington, Canada | Mike Brcic | ink.inkblottherapy.com |
| InnerlogicB2B | B2B | Bedford, Canada | Jordan Friesen | innerlogic.com |
| InnvoxB2B | B2B | Coral Springs, United States | — | innvox.com |
| InphluB2B | B2B | Cincinnati, United States | — | lunchtable.ai |
| Inquisio.AIB2B | B2B | Orting, United States | — | inquisio.ai |
| Insent.aiB2B | B2B | Littleton, United States | Prayag Narula | insent.ai |
| INSPECTORIOB2B | B2B | Minneapolis, United States | Carlos Moncayo | inspectorio.com |
| InstaLabelB2B | B2B | New York City, United States | — | instalabel.ai |
| InStrydeOther | Other | Winston-Salem, United States | — | instryde.com |
| InsureTaxFintech | Fintech | Tel Aviv, Israel | — | insure.tax |
| IntelitalB2B | B2B | Hallandale Beach, United States | — | intelital.com |
| IntelizenB2B | B2B | Philadelphia, United States | — | intelizen.co |
| IntellectibleB2B | B2B | Austin, United States | — | intellectible.com |
| IntelligemsOther | Other | Denver, United States | Matt Bahr | intelligems.io |
| IntenseDebateOther | Other | Boulder, United States | Matt Mullenweg | intensedebate.com |
| Interface FluidicsIndustrials | Industrials | Calgary, Canada | David Sinton | interfacefluidics.com |
| InthegameB2B | B2B | Tel Aviv, Israel | — | inthegame.io |
| IntuosIndustrials | Industrials | L Aquila, Italy | — | intuos.it |
| INURUIndustrials | Industrials | Berlin, Germany | — | inuru.de |
| InvendorOther | Other | Lehmja küla, Estonia | — | invendor.com |
| Involve.AIOther | Other | Santa Monica, United States | Gaurav Bhawnani | involvesoft.com |
| InvomaticB2B | B2B | Bogotá, Colombia | — | innapsis.com.co |
| IomobFintech | Fintech | Tallinn, Estonia | Boyd Cohen | iomob.net |
| IONAIndustrials | Industrials | Bedford, United Kingdom | — | ionadrones.com |
| IonicB2B | B2B | Madison, United States | Max Lynch | ionic.io |
| IOpipeOther | Other | Seattle, United States | Adam Johnson | iopipe.com |
| IQID TechnologyEducation | Education | Lewes, United States | — | iqid.io |
| IrisMedB2B | B2B | Aurora, United States | — | irismed.co |
| IronCore LabsB2B | B2B | Boulder, United States | Patrick Walsh | ironcorelabs.com |
| IrysConsumer | Consumer | San Antonio, United States | — | irystechnologies.com |
| It's By UIndustrials | Industrials | New York City, United States | — | itsbyu.com |
| It's JulyIndustrials | Industrials | Tel Aviv, Israel | — | itsjuly.com |
| ithnainHealthcare | Healthcare | Riyadh, Saudi Arabia | — | ithnain.com |
| Jaipur RoboticsOther | Other | Manno, Switzerland | — | jaipurrobotics.com |
| Jam LifeOther | Other | Encino, United States | — | jamfamilycalendar.com |
| JazzyPayFintech | Fintech | Makati, Philippines | — | jazzypay.com |
| JebbitOther | Other | Boston, United States | Tom Coburn | jebbit.com |