| EnertivB2B | B2B | New York City, United States | Connell McGill | enertiv.com |
| EneryieldB2B | B2B | Göteborg, Sweden | — | eneryield.com |
| EnjoyHQB2B | B2B | London, United Kingdom | Roman Kasavchenko | getenjoyhq.com |
| ENSOIndustrials | Industrials | San Francisco, United States | — | enso.de |
| EnurgenIndustrials | Industrials | Ottawa, Canada | — | enurgen.com |
| eolaFintech | Fintech | London, United Kingdom | — | eola.co |
| Ephemeral SolutionsIndustrials | Industrials | New York City, United States | Josh Sakhai | ephemeraltattoos.com |
| EpicurateOther | Other | Dundee, United States | — | epicurate.vip |
| EQL FinanceB2B | B2B | Newark, United States | — | eqlfinance.com |
| EqualiFintech | Fintech | London, United Kingdom | — | equali.io |
| Equipment ConnectOther | Other | London, United Kingdom | — | equipal.co |
| EquoIndustrials | Industrials | Ho Chi Minh City, Vietnam | Giacomo Piva | shopequo.com |
| ErgattaOther | Other | Brooklyn, United States | Tom Aulet | ergatta.com |
| Esper Satellite ImageryOther | Other | Patterson Lakes, Australia | — | espersatellites.co |
| EstatedB2B | B2B | Kelowna, Canada | Brandon Donnelly | estated.com |
| Et Cetera RoboticsOther | Other | Seattle, United States | — | etcrobotics.com |
| Etiq AIFintech | Fintech | London, United Kingdom | Larysa Visengeriyeva | etiq.ai |
| EventnoireOther | Other | Chicago, United States | Tahjee Moore | eventnoire.com |
| EverledgerOther | Other | London, United Kingdom | Leanne Kemp | |
| EversendOther | Other | Paris, France | Stone Atwine | eversend.co |
| EversportConsumer | Consumer | Vienna, Austria | Herwig Marbler | eversport.at |
| EverTrueConsumer | Consumer | Cambridge, United States | Brent Grinna | evertrue.com |
| Every MotherOther | Other | Brooklyn, United States | Diastasis Recti founder — Leah Keller | every-mother.com |
| Everyday Life InsuranceFintech | Fintech | Andover, United States | Peter Colis | everydaylifeinsurance.com |
| EvrimB2B | B2B | Norfolk, United States | — | evrim.ai |
| eWibeOther | Other | Milano, Italy | — | ewibe.com |
| ExordiB2B | B2B | London, United Kingdom | — | exordi.io |
| Experiment EngineOther | Other | Austin, United States | Alexis Maybank | experimentengine.com |
| EyeLightsOther | Other | Labège, France | Antoine Tardy | eye-lights.com |
| f3niceIndustrials | Industrials | Milan, Italy | — | f3nice.com |
| FableIndustrials | Industrials | Vancouver, Canada | Veronika Harbick | us.fablehome.co |
| FabricaFintech | Fintech | San Francisco, United States | Brian Frumberg | fabrica.land |
| FabriSightOther | Other | Vancouver, Canada | — | tryfabrisight.com |
| FaibleB2B | B2B | Las Vegas, United States | — | site.mythia.ai |
| FairsplitFintech | Fintech | Miami, United States | — | fairsplit.us |
| Family FirstIndustrials | Industrials | Boston, United States | — | family-first.com |
| FanaplyConsumer | Consumer | Huntington, United States | — | fanaply.com |
| FanbaseOther | Other | Edinburgh, United Kingdom | Isaac Hayes III | fanbaseclub.com |
| FanSifterB2B | B2B | Tallinn, Estonia | — | fansifter.com |
| FantasmoConsumer | Consumer | Los Angeles, United States | Jameson Detweiler | fantasmo.io |
| FarmCrowdyConsumer | Consumer | Lagos, Nigeria | Onyeka Akumah | farmcrowdy.com |
| Farmz2UFintech | Fintech | Lagos, Nigeria | — | farmz2u.com |
| Fasten HealthHealthcare | Healthcare | San Francisco, United States | Brad Van Leeuwen | fastenhealth.com |
| FaveB2B | B2B | Dietikon, Switzerland | Jacqueline Ros | faveforfans.com |
| FeezBackFintech | Fintech | Tel Aviv, Israel | — | feezback.com |
| FelixB2B | B2B | Dubai, United Arab Emirates | — | liberationcover.com |
| FelixFusionOther | Other | Roseville, United States | — | felixfusion.ai |
| FEMOther | Other | San Francisco, United States | — | fem-inc.com |
| FEYENALLYB2B | B2B | Wilmington, United States | — | feyenally.com |
| FibrXOther | Other | Henderson, United States | — | fibrx.io |