| DragApp MASTERConsumer | Consumer | Royston, United Kingdom | Roger Baptista Lara | dragapp.com |
| DriverOther | Other | Brooklyn, United States | — | trydriver.com |
| DrugviuHealthcare | Healthcare | Chicago, United States | — | viuhealth.com |
| DwellSocialOther | Other | Northbrook, United States | — | dwellsocial.com |
| Dynamic Green ProductsOther | Other | Houston, United States | — | changeyouroil.com |
| DynepicOther | Other | Folly Beach, United States | John Saffrett | dynepic.com |
| DyrtIndustrials | Industrials | Rancho Palos Verdes, United States | Sarah Smith | dyrt.co |
| E-FishIndustrials | Industrials | Newport Beach, United States | — | e-fish.com |
| EA Global AIB2B | B2B | St Briavels, United Kingdom | — | eaglobal.ai |
| EarBudsConsumer | Consumer | Austin, United States | Alexa Binns | earbudsmusic.com |
| Easy PlatterOther | Other | Halifax, Canada | — | easyplatter.com |
| EasyWay TechnologiesOther | Other | Tel Aviv, Israel | — | easyway.ai |
| Echo LabsB2B | B2B | San Francisco, United States | — | el.ai |
| echo3DOther | Other | New York City, United States | Alon Grinshpoon | echo3d.co |
| ECO2GROWIndustrials | Industrials | Bonn, Germany | — | eco2grow.com |
| EcoGB2B | B2B | Munich, Germany | Gregor Adamczyk | ecog.io |
| EcolectroIndustrials | Industrials | Ithaca, United States | Minette Lagnes | ecolectro.com |
| EcolyticsB2B | B2B | Durham, United States | — | ecolytics.io |
| EcoMap TechnologiesOther | Other | Wilmington, United States | — | ecomap.tech |
| EcomtentB2B | B2B | Toronto, Canada | Max Sinclair | ecomtent.ai |
| EcoPlantOther | Other | Ashqelon, Israel | Yotam Ariel | ecoplant.co |
| Eddy TravelsB2B | B2B | Toronto, Canada | Julius Str | eddytravels.com |
| EDGE Sound ResearchOther | Other | Riverside, United States | — | edgesoundresearch.com |
| Edify TechnologiesB2B | B2B | Boulder, United States | Josh Silverman | edify.co |
| edisn.aiB2B | B2B | Chandler, United States | — | edisn.ai |
| edison.aiConsumer | Consumer | Tokyo, Japan | Mikael Berner | edison.ai |
| Edith LabsFintech | Fintech | San Carlos, United States | — | edithlabs.com |
| EdSightsB2B | B2B | New York City, United States | Ashton Cofer | edsights.io |
| EducativeEducation | Education | Bellevue, United States | Fahim ul Haq | educative.io |
| Edvisor.ioOther | Other | Vancouver, Canada | — | edvisor.io |
| EINOB2B | B2B | Brooklyn, United States | — | eino.ai |
| ELANZA WellnessOther | Other | San Francisco, United States | — | elanzawellness.com |
| Elastic AudioConsumer | Consumer | Decatur, United States | — | elasticaudio.com |
| ElectricFishIndustrials | Industrials | Fremont, United States | — | electricfish.co |
| Elegus TechnologiesIndustrials | Industrials | Ann Arbor, United States | Aaron Alvarez | elegustech.com |
| Elektrik AppIndustrials | Industrials | Lindon, United States | — | elektrikapp.com |
| EliB2B | B2B | Montréal, Canada | — | eli.health |
| Elio.EarthB2B | B2B | Yorba Linda, United States | — | elio.earth |
| Elite Personal ChefsOther | Other | Chicago, United States | — | elitepersonalchefs.com |
| Ellipsis DriveOther | Other | Ede, Netherlands | — | ellipsis-drive.com |
| ElmetrOther | Other | Alexandria - aba shark, United States | — | elmetr.com |
| ELV8Fintech | Fintech | New York City, United States | — | investwithscout.com |
| Email CopilotB2B | B2B | San Diego, United States | — | emailcopilot.com |
| EmbeddableOther | Other | Berlin, Germany | Frank Fradella | trevor.io |
| EmbodyMeB2B | B2B | Tokyo, Japan | — | company.embodyme.com |
| Emission Free GeneratorsIndustrials | Industrials | Covina, United States | — | emissionfreegenerators.com |
| EMOTIVB2B | B2B | San Francisco, United States | Tan Le | emotiv.com |
| EmpalloB2B | B2B | Cambridge, United States | — | empallo.com |
| Employee CycleB2B | B2B | Philadelphia, United States | Ryan Engel | employeecycle.com |
| EndelB2B | B2B | Berlin, Germany | Oleg Stavitsky | endel.io |